plenti hreluc pgk hygro Search Results


plenti hreluc pgk hygro  (Addgene inc)
92
Addgene inc plenti hreluc pgk hygro
Figure 3. Stable expression of exogenous human FXN in MUT MEFs (A) A schematic of the lentivirus vector <t>pLenti</t> <t>hygro</t> FXN encoding a human miniFXN gene. The miniFXN gene used in experiments contains fragments of the endogenous FXN 50UTR, promoter and intron 1, and all five exons of the gene. (B) Representative western blot demonstrating expression of exogenous FXN. The miniFXN encodes a C-terminal HA tag that results in a slightly greater molecular weight, as detected by western blot. (C) Quantitation of endogenous Fxn (*) and exogenous FXN by western blot. Gapdh was used as a normalization control. MUT+FXN designates cells expressing exogenous, human FXN. The experiment was performed three independent times. Results are plotted as mean values with standard deviations; (**p < 0.001, ***p < 0.0001).
Plenti Hreluc Pgk Hygro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plenti+hreluc+pgk+hygro/pLenti+HRE-Luc+pGK+Hygro+(Plasmid+%23118706)/pm39823061-164-24-28
Average 92 stars, based on 1 article reviews
plenti hreluc pgk hygro - by Bioz Stars, 2026-10
92/100 stars
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reporter hreluc  (Addgene inc)
94
Addgene inc reporter hreluc
Figure 3. Stable expression of exogenous human FXN in MUT MEFs (A) A schematic of the lentivirus vector <t>pLenti</t> <t>hygro</t> FXN encoding a human miniFXN gene. The miniFXN gene used in experiments contains fragments of the endogenous FXN 50UTR, promoter and intron 1, and all five exons of the gene. (B) Representative western blot demonstrating expression of exogenous FXN. The miniFXN encodes a C-terminal HA tag that results in a slightly greater molecular weight, as detected by western blot. (C) Quantitation of endogenous Fxn (*) and exogenous FXN by western blot. Gapdh was used as a normalization control. MUT+FXN designates cells expressing exogenous, human FXN. The experiment was performed three independent times. Results are plotted as mean values with standard deviations; (**p < 0.001, ***p < 0.0001).
Reporter Hreluc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plenti+hreluc+pgk+hygro/HRE-luciferase+(Plasmid+%2326731)/pm40595592-336-17-19
Average 94 stars, based on 1 article reviews
reporter hreluc - by Bioz Stars, 2026-10
94/100 stars
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Figure 3. Stable expression of exogenous human FXN in MUT MEFs (A) A schematic of the lentivirus vector pLenti hygro FXN encoding a human miniFXN gene. The miniFXN gene used in experiments contains fragments of the endogenous FXN 50UTR, promoter and intron 1, and all five exons of the gene. (B) Representative western blot demonstrating expression of exogenous FXN. The miniFXN encodes a C-terminal HA tag that results in a slightly greater molecular weight, as detected by western blot. (C) Quantitation of endogenous Fxn (*) and exogenous FXN by western blot. Gapdh was used as a normalization control. MUT+FXN designates cells expressing exogenous, human FXN. The experiment was performed three independent times. Results are plotted as mean values with standard deviations; (**p < 0.001, ***p < 0.0001).

Journal: Molecular therapy. Methods & clinical development

Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments.

doi: 10.1016/j.omtm.2024.101347

Figure Lengend Snippet: Figure 3. Stable expression of exogenous human FXN in MUT MEFs (A) A schematic of the lentivirus vector pLenti hygro FXN encoding a human miniFXN gene. The miniFXN gene used in experiments contains fragments of the endogenous FXN 50UTR, promoter and intron 1, and all five exons of the gene. (B) Representative western blot demonstrating expression of exogenous FXN. The miniFXN encodes a C-terminal HA tag that results in a slightly greater molecular weight, as detected by western blot. (C) Quantitation of endogenous Fxn (*) and exogenous FXN by western blot. Gapdh was used as a normalization control. MUT+FXN designates cells expressing exogenous, human FXN. The experiment was performed three independent times. Results are plotted as mean values with standard deviations; (**p < 0.001, ***p < 0.0001).

Article Snippet: Cloning and packaging of pLenti hygro FXN-L A miniFXN construct expressing human FXN under the control of endogenous regulatory elements was cloned into the pLenti HRELuc pGK Hygro (Addgene plasmid #118706).

Techniques: Expressing, Plasmid Preparation, Western Blot, Molecular Weight, Quantitation Assay, Control